Bromothymol blue is used as a pH indicator - starting color around pH 6.8 is green. So why does Simmons Citrate change its color from green to royal blue? This is because alongside citrate, which gets converted into pyruvic acid and some other end products, the medium contains ammonium salts that produce ammonia and ammonium hydroxide upon their utilization thus resulting in an increase in alkalinity and a color change in the pH color indicator.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment